Simultaneous Determination of Two Specific Flavonoid Glycosides in Lysimachia Capillipes Hemsl. by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination of Quercetin-3-O-(2'', 6''-di-O- α-rhamnopyranosyl)-β-galactopyranoside and Kaempferol-3-O-2-glucopyranosyl(1→3)rhamnopyranosyl-6-rhamnopyranosyl- β-D-galactopyranoside in Lysimachia capillipes Hemsl. METHODS The HPLC analysis was carried on SHIMADZU Shim- pack VP-ODS C₁₈ column(250 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile-0.3% phosphoric acid aqueous solution (gradient elution) at the flow rate of 1 mL·min^-1. The column temperature was set at 30℃, and the detection wavelength was set at 350 nm. RESULTS Quercetin-3-O-(2'',6''-di-O-α-rhamnopyranosyl)-β-galactopyranoside and Kaempferol-3-O-2- glucopyranosyl(1→3)rhamnopyranosyl-6-rhamnopyranosyl-β-D-galactopyranoside were showed good linear relationships within the ranges of 0.041 46-2.072 8 μg·mL^-1(r=0.999 9) and 0.088 95-4.447 5 μg·mL^-1(r=0.999 9). The average recoveries (n=6) were 98.19% and 98.85%, RSDS were 1.94% and 1.81%, respectively. CONCLUSION The established method is simple, specific, and suitable for simultaneous determination of two components of specific flavonoid glycosides in Lysimachia capillipes Hemsl.