Effect of Kaempferol on the Proliferation of Non-small Cell Lung Cancer A549 Cells by Regulating miR21

OBJECTIVE To study the inhibitory effect of kaempferol(KA) on the proliferation of non-small cell lung cancer A549 cells and its mechanism. METHODS CCK-8 cell viability assay and colony formation assay were used to study the effect of KA on the survival rate of A549 cells and determine the treatment concentration of KA. A549 cells were divided into control group and different doses of KA group, and the effects of different concentrations of KA on the expression of microRNA in A549 cells were detected by qPCR. A549 cells were transfected by miR21 mimc. Overexpression of miR21 in A549 cells was detected by qPCR. The effects of miR21 and KA on PTEN/AKT signaling pathway were detected by Western blotting and qPCR. RESULTS CCK-8 and colony formation assays demonstrated that KA could inhibit the proliferation of non-small cell lung cancer A549 cells. KA treatment for 24 h at 30, 90 μmol·L^-1 obviously reduced the expression of miR21 and p-AKT, c-Myc, Cyclin D1 in A549 cells, and increased the expression of PTEN. However, the expression of p-AKT, c-Myc and Cyclin D1 was upregulated and PTEN was downregulated after miR21 mimc transfection. Finally the rescue experiment confirmed that KA induced inhibitory proliferation effect of A549 cells could be blocked by miR21 mimc transfection. CONCLUSION KA can inhibit the proliferation of non-small cell lung cancer A549 cells via regulating the miR21/PTEN/AKT signaling pathway.