Study on effect of tail-amputated earthworm on Contents of inorganic elements in wound Skin of rats by ICP-MS

OBJECTIVE To establish method for the content determination of inorganic elements in wound skin of rats by ICP-MS with microwave digestion, and to investigate the relationship between the effect of tail-amputated earthworm on wound healing and inorganic elements. METHODS The samples were pre-treated by HNO₃ system-microwave digestion method, and 14 inorganic elements including K, Mg, Ca, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Cd, Te and Pb were determined and analyzed by ICP-MS. RESULTS The method showed good linearity at 0-250 μg·L^-1(r ≥ 0.999) under optimized experimental conditions. The limit of detection (3σ, n=10) was 0.02-13.04 μg·L^-1. The recovery(n=6) was 85.4%-119.0%. The content of K, Ca, Mg, Cu, Fe, Zn and Se in the wound tissue was obviously increased by tail-amputated without increasing the content of heavy metal elements. CONCLUSION This method is simple, rapid, sensitive and accurate, and it can be used for simultaneous determination of inorganic elements on wound skin of rats. Tail-amputated earthworm plays a role in promoting wound healing, which is related to increasing the content of inorganic elements and has a higher safety.