Simultaneous Determination of Borneol, Isoborneol and Muscone in Shexiang Tongxin Dripping Pills by GC

OBJECTIVE To establish a GC method for the simultaneous determination of borneol, isoborneol and muscone in Shexiang Tongxin Dripping Pills. METHODS Sample of Shexiang Tongxin Dripping pills were determined by HP-5MS capillary column(30 m×0.32 mm, 0.25 μm). FID was used as detector with a temperature of 250℃, and the inlet temperature was 220℃. The column temperature was programmed to start at 100℃ maintained for 2 min, rised to 110℃ at a rate of 2℃·min^-1, maintained for 3 min, and then rised to 205℃ at a rate of 50℃·min^-1, maintained for 7 min, and then rised to 250℃ at a rate of 20℃·min^-1 maintained for 2 min. Nitrogen(1 mL·min^-1) was used as the carrying gas, and the split ratio was 10:1. The injection volume was 1 μL and naphthalene was used as internal standard. RESULTS The linear ranges of borneol, isoborneol and muscone were 0.054 68-0.437 4 mg·mL^-1(r=0.999 9), 0.070 67-0.565 4 mg·mL^-1(r=0.999 9), 0.004 512-0.036 10 mg·mL^-1(r=0.999 7), respectively. The average recovery rates(n=6) were 97.91%(RSD=2.05%), 100.79% (RSD=2.78%), 105.36%(RSD=3.78%), respectively. CONCLUSION This method is reliable and accurate, which can be used for the determination of borneol, isoborneol and muscone in Shexiang Tongxin Dripping Pills.