Fingerprint of Paeoniae Radix Alba Standard Decoction from Different Producing Areas and Determination of Four Monoterpenoid Glycosides Components

OBJECTIVE To establish a quality evaluation method for standard decoctions of Paeoniae Radix Alba from different producing areas, and to analyze the differences of Paeoniae Radix Alba decoction pieces from different producing areas. METHODS According to the preparation requirements of standard decoctions, 15 batches of standard decoctions of Paeoniae Radix Alba from different producing areas were prepared. HPLC was used to establish fingerprint. Meanwhile, the contents of oxypaeoniflorin, albiflorin, paeoniflorin and benzoyl paeoniflorin as well as the transfer rate and the extraction rate of standard decoctions were determined. Variance analysis was carried out on all samples based on the content determination results of standard decoctions. RESULTS The extraction rate of 15 batches of Paeoniae Radix Alba standard decoctions from different producing areas ranged from 15.39% to 27.81%, the average extraction rate was 23.15%, and the standard deviation was 4.44%. The content range of oxypaeoniflorin was 0.015%-0.046%, and the transfer rate was 70.04%-129.26%. The content range of albiflorin was 0.315%-1.150%, and the transfer rate was 70.77%-119.80%. The content range of paeoniflorin was 1.857%-2.522%, and the transfer rate was 67.84%-99.16%. The content range of benzoyl paeoniflorin was 0.051%-0.172%, and the transfer rate was 56.11%-88.00%. The fingerprint included 14 peaks and 4 calibrations, and the similarity was >0.97. CONCLUSION The components of Paeoniae Radix Alba and standard decoctions from different producing areas are basically the same. Paeoniflorin and benzoylpaeoniflorin in standard decoctions of Paeoniae Radix Alba have no difference, while oxypaeoniflorin and albiflorin have difference.