Fingerprint and Multi-component Quantification Analysis of Salvia Miltiorrhiza Extract Intermediate from Xutong Injection

OBJECTIVE To establish the HPLC-UV fingerprint of Salvia miltiorrhiza extract intermediate from Xutong injection, and simultaneously to determine the contents of 7 indicators including 5-HMF, Danshensu, protocatechualdehyde, rosmarinic acid, lithospermic acid, salvianolic acid A, salvianolic acid B. METHODS The separation was achieved on a Waters Cortecs C₁₈ column(100 mm×4.6 mm, 2.7 μm) with a flow rate of 0.8 mL·min^-1 in line gradient mode at 25℃. The 0.4% formic acid solution-0.4% formic acid acetonitrile were used as mobile phases A and B, respectively. The sample injection volume was set at 3 μL and detection wavelength was 280 nm. RESULTS The established fingerprint with good resolution and symmetrical peak shape could comprehensively reflect the chemical information of Salvia miltiorrhiza extract. The 24 common peaks were identified and the fingerprint similarity among 10 batches of extract was >0.960. Within a certain mass range, the peak area and mass concentration of 7 indicators had a good linear relationship. The average recovery of 7 kinds of compounds was 96.49%-100.18% and the RSD was 1.24%-2.27%(n=9), the total amount of the 7 indicators in the 10 batches of Salvia miltiorrhiza extract ranged was 8 202.2-12 078.7 μg·g^-1. CONCLUSION The established HPLC-UV method is accurate, simple and reproducible. HPLC-UV fingerprint combined with quantitative determination of indicators can more fully reflect the quality of Salvia miltiorrhiza extract to provide reference for quality evaluation of Salvia miltiorrhiza extract immediate from Xutong injection.