Simultaneous Determination of Succinic Acid, Hesperidin and Liquiritin in Banxia Syrup by HPLC

OBJECTIVE The high performance liquid chromatography(HPLC) method was established to simultaneously determine the level of succinic acid, hesperidin and liquiritin in Banxia syrup. METHODS The C₁₈ chromatographic column (4.6 mm×150 mm, 5 μm) was adopted, acetonitrile-0.2% phosphoric acid solution(18:82) was applied as mobile phase with flow rate of 1.0 mL·min^-1 under the wavelength of 207 nm. The column temperature was room temperature. RESULTS The compositions were separated perfectly without interference from negative controls. The liner range of Succinic acid, Hesperidin and liquiritin were 4.727-118.17 μg·mL^-1(r=0.999 9), 2.474-61.857 μg·mL^-1(r=0.999 6) and 2.469-61.725 μg·mL^-1(r=0.999 9), respectively. The average recovery rates of analytes(n=9) were 100.8%, 99.3% and 100.2% with their RSD value of 1.3%, 1.2% and 1.8%. The range level of succinic acid, hesperidin and liquiritin in Banxia syrup were 0.072 2-0.079 4 mg·mL^-1, 0.029 9-0.034 8 mg·mL^-1 and 0.022 8-0.029 0 mg·mL^-1. CONCLUSION It simplifies the composition analysis, reduces experiment time and ensures the repeatability, which may be used as an effective method for the quality control of Banxia syrup.