Simultaneous Determination of 4 Components in Buddleja Officinalis Maxim. from Different Regions by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of acteoside, linarin, apigenin, luteolin in Buddleja officinalis Maxim. and to compare the content of Buddleja officinalis Maxim. from different origins. METHODS The analysis was performed on Waters Sunfire^TM C₁₈ analytical column(4.6 mm×250 mm, 5 µm) with the gradientelution of acetonitrile-0.05% phosphoric acid solution at a flow rate of 1.0 mL·min^-1, the column temperature was 30℃ and the detection wavelength was set at 330 nm(acteoside, linarin, apigenin) and 350 nm(luteolin). RESULTS Four constituents showed good linear relationships(r>0.999 0) within their own ranges, whose average recoveries were 97.75%-98.49% with the RSDs of 0.7%-1.5%. The contents of acteoside, linarin, apigenin, luteolin in samples of different regions were in ranges of 21.046 3-42.886 3, 8.514 4-17.400 8, 0.317 3-1.205 3, 0.030 4-0.145 6, respectively. CONCLUSION This reliable, stable and reproducible method can be used to evaluate the quality of Buddleja officinalis Maxim..