Determination of Astragaloside Ⅳ, Catalpol and Acteoside in Shenkangling Granules by HPLC-DAD-ELSD

OBJECTIVE To establish a method for the determination of astragaloside Ⅳ, catalpol and acteoside in Shenkangling granules, and to provide an experimental reference for the quality control of Shenkangling granules. METHODS The contents of astragaloside Ⅳ, catalpol and acteoside in the granules were detected by HPLC-DAD-ELSD with a Agilent EC-C₁₈ column(4.6 mm×250 mm, 5 μm), a mobile phase was acetonitrile-water in a gradient elution manner, velocity of flow was 1.0 mL·min^-1, the temperature of column was 30℃, and detection wavelength was set at 210 nm. The temperature of drift tube was set at 80℃, and the gas flow was set at 1.5 L·min^-1. RESULTS Astragaloside Ⅳ, catalpol and acteoside displayed good linear relationships within the range of 22.82-456.3 μg·mL^-1(r=0.999 9), 17.83-356.6 μg·mL^-1(r=0.999 8), 11.36-227.2 μg·mL^-1(r=0.999 9). And the average recoveries were 100.39%(RSD=1.0%), 100.79%(RSD=1.2%), 100.07% (RSD=0.43%) (n=9), respectively. CONCLUSION The contents of 3 components were simultaneous detected. The method is simple, rapid, accurate and reproducible. It can be used as an effective method to evaluate the internal quality of Shenkangling granules.