Pharmacodynamics Evaluation of Taxus Chinensis Extract on Chronic Obstructive Pulmonary Disease Mice

OBJECTIVE To observe the effect of Taxus chinensis extract on chronic obstructive pulmonary disease(COPD) mice, evaluate its efficacy, and study its mechanisms. METHODS Smoke mice model for COPD was established, and the broncho alveolar lavage fluid(BALF) and lung tissues were finally collected for total differential white blood cells counting and HE and AB/PAS staining. The SOD, GSH, MPO activity, as well as TNF-a, KC and MIP-2 expression were determined to evaluate inflammatory infiltration. RESULTS The extract of Taxus chinensis significantly decreased the content of WBC, increased the activity of SOD and GSH, and inhibited MPO activity and TNF-a and KC inflammatory factors. CONCLUSION The extract of Taxus chinensis show effectiveness on COPD mice model, with its mechanism of action associated with inhibition of migration of neutrophil, the generation of inflammatory cytokines and mucus secretion of airway.