Simultaneous Determination of Nine Compositions in Chenxiang Shuqi Wan by HPLC Wavelength Switching Method

OBJECTIVE To develop and validate an HPLC wavelength switching method for detemining contents of α-cyperone, costunolide, dehydrocostuslactone, protopine, tetrahydropalmatine, corydaline, tetrahydroberberine, honokiol and magnolol in Chenxiang Shuqi Wan. METHODS HPLC method was employed on a Kromasil Eternity C₁₈ chromatographic column(250 mm×4.6 mm, 5 µm). The mobile phase was methanol-acetonitrile(1：1)(A)-0.1% phosphoric acid solution(B) with gradient elution. The detection wavelengths were 250, 225, 280, 294 nm. The flow rate was 0.9 mL·min^-1, and the column temperature was 30 ℃. RESULTS The liner ranges of α-cyperone, costunolide, dehydrocostuslactone, protopine, tetrahydropalmatine, corydaline, tetrahydroberberine, honokiol and magnolol were within 5.79-115.80, 7.86-157.20, 9.68-193.60, 1.07-21.40, 3.09-61.80, 3.77-75.40, 2.09-41.80, 15.77-315.40, 19.86-397.20 μg·mL^-1. The average recovery rates were 97.95%, 98.97%, 99.46%, 97.37%, 98.48%, 98.10%, 96.90%, 99.35% and 100.10%, with RSD of 1.35%, 1.17%, 0.89%, 1.57%, 1.27%, 0.96%, 1.09%, 0.74% and 0.90%. CONCLUSION The method was easy to operate and repeatable, can be used for quality control of Chenxiang Shuqi Wan．