Study on Fingerprint and Multi-components Determination of Niuhuang Qinggan Capsules by HPLC

OBJECTIVE To establish the HPLC fingerprint and determine the contents of seven compounds in Niuhuang Qinggan capsules simultaneously. METHODS The separation was carried out on an Agilent SB-C₁₈ column(250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile(A)-0.1% acetic acid solution(B) with gradient elution, the detection wavelength was 210 nm, and column temperature was 30℃. "Similarity evaluation system for chromatographic fingerprint of TCM (2004A)" was used to conduct, and the seven markers were quantified. RESULTS The common mode of the fingerprint showed 22 common peaks, 7 of which were identified by comparison with the reference substances. They were chlorogenic acid, caffeic acid, luteolin-7-o-glucoside, baicalin, baicalein, wogonoside and wogonin. The similar degrees of 12 batches of samples were > 0.90, and the average recoveries varied from 99.1%-104.8% with RSDs of 1.30%-1.88%. CONCLUSION The established method can be used for the quality control of Niuhuang Qinggan capsules.