Establishing Fingerprint of the Leaves of Dendropanax Dentiger Based on LC-MS

OBJECTIVE To establish the HPLC fingerprint of the leaves of Dendropanax dentiger based on LC-MS. METHODS The column was Waters BEH C₁₈(2.1 mm×100 mm, 1.7 μm). Mobile phase consisted acetonitrile(A)-0.1% formic acid(B) with gradient elution. Column temperature was 35℃, flow rate was 0.2 mL·min^-1 and injection volume was 1 mL. Mass spectrum condition was electrospray ionization source with negative ion detection, and the source temperature was 150℃, the desolvation temperature was 500℃, the desolvation with N₂ was 800 L·h^-1, the cone with N₂ was 50 L·h^-1, the collision gas was Ar, the capilary was 3 000 V. HPLC with Waters Sunfire^TM C₁₈(4.6 mm×250 mm, 5 μm) column was used. Mobile phase consisted acetonitrile(A)-aqueoussolution(B) including 0.2% formic acid and 0.06% tetrahydrofuran with gradient elution. Detection wavelength was 256 nm. Column temperature was 30℃. Flow rate was 1.0 mL·min^-1 and analytic time was 80 min. Injection volume was 5 μL. Used the methods established above to be the HPLC fingerprint of Dendropanax dentiger. RESULTS Under the same fingerprint chromatogram condition, the methodological results were satisfactory to 10 batches of the samples. And the similarity range was 0.939-0.989. CONCLUSION Dendropanax dentiger is an evergreen plant. The chemical constituents in its leaves are closely related to the growth years, environment and harvesting season of the plant. The established fingerprint identification method is simple and reproducible, which can provide a scientific basis for the quality evaluation of the leaves of Dendropanax dentiger.