Study on Regulation Effect of Troxipide on Gastric Ulcer Induced by Acetic Acid in Rats

OBJECTIVE To investigate the efficacy and mechanism of troxipide on treatment of chronic gastric ulcer induced by acetic acid. METHODS Using chronic acetic acid to establish gastric ulcer model, the successful model of the rats were randomly divided into model group, ranitidine group, high dose and low dose group of troxipide. Each group had 10 rats, another 10 healthy rats were set as a blank control group. After 14 d of model, the drugs were given to rats daily for 7 d by intragastric administration and then executed. The ulcer area of rats in each group were measured, the ulcer index and inhibition rate were calculated. Histopathological morphology was observed by HE, the levels of IL-1, IL-6, NF-κB, TNF-α, PGE2, MDA, SOD activity and the relative content of oxygen free radicals were detected in gastric tissue. The mRNA expression of EGF, VEGF, TNF-α, NF-κB in gastric tissue were examined. RESULTS Compared with the model group, ulcer area and ulcer index were significantly improved(P<0.05 or P<0.01). The pathological changes of gastric tissue were improved in different degree; the levels of IL-1, IL-6, NF-κB, TNF-α, PGE2, MDA activity and the relative content of oxygen free radicals in gastric tissue reduced significantly, and SOD was significantly increased(P<0.05 or P<0.01); the mRNA levels of EGF, VEGF, TNF-α and NF-κB in gastric tissue were significantly improved(P<0.05 or P<0.01). CONCLUSION PT has an effectively effect on gastric ulcer, and its mechanism may be related to promoting the proliferation and differentiation of angiogenesis, epithelial and marginal epidermal cells in the gastrointestinal mucosa of gastric ulcer rats, and the role of eliminating inflammatory factors.