Effect of Nano-MK-ASODN in Orthotopic Implantation of Human Hepatocellular Carcinoma

OBJECTIVE To investigate the inhibition effect of midkine antisense oligodeoxynucleotides(Nano-MK-ASODN) on the orthotopic transplantation model of human hepatocellular carcinoma in nude mice. METHODS Nude mice with model of human hepatocellular carcinoma were divided into solvent control group, liposome control group, nonsense-ASODN control, 25, 12.5 and 6.25 mg·kg^-1 of MK-ASODN group, 25, 12.5 and 6.25 mg·kg^-1 of Nano-MK-ASODN group. Normal nude mice were selected as normal control group. Weight, tumor weight, tumor growth inhibition rate, blood routine and AFP of nude mice were detected. RESULTS The tumor suppression rates of MK-ASODN 25, 12.5, 6.25 mg·kg^-1 group were 53.72%, 48.76%, 42.98%, the tumor suppression rates of Nano-MK-ASODN 25, 12.5, 6.25 mg·kg^-1 were 66.94%, 56.20%, and 52.07%. The tumor quality of each group was significantly different from that of the solvent control group(P<0.05). The tumor mass and AFP level in the Nano-MK-ASODN group were significantly lower than those in the solvent control group (P<0.05). There was no significant difference in the body weight, peripheral blood routine, between Nano-MK-ASODN group and liposome control group, nonsense-ASODN control or normal control group. At the same time, anatomical and histopathological examination showed that the volume of liver tumor in Nano-MK-ASODN treated group was reduced and liquefied, which could cause degeneration and necrosis of some hepatocellular carcinoma cells. CONCLUSION Nano-MK-ASODN could significantly inhibit human hepatocellular carcinoma orthotopic transplantation in nude mice, and the effect was significantly stronger than that of MK-ASODN.