Simultaneous Determination of Nine Components in Xiongju Shangqing Pills by UHPLC Combined with Chemical Pattern Recognition

OBJECTIVE To develop a UHPLC method for the simultaneous determination of 9 components in Xiongju Shangqing pills. METHODS An Agilent Ecilipse C₁₈(2.1 mm×100 mm, 1.6 μm) chromatographic column was used; the mobile phase was methanol-0.05% phosphoric acid solution with gradient elution at the flow rate of 0.3 mL·min^-1 and the detection wavelengths were 327, 237, 320, 345, 278, 254 nm and the column temperature was 30℃, the injection volume was 2 μL. The principal component analysis(PCA) and cluster analysis(CA) were conducted for the results of content determination by SPSS 22.0 statistical software. RESULTS The linear ranges of chlorogenic acid, 3,5-dicaffeoylquinic acid, geniposide, liquiritin, ferulic acid, berberine hydrochloride, baicalin, prim-O-glucosylcimifugin, 5-O-methylvisammioside were 4.30-68.80 μg·mL^-1 (r=0.999 0), 6.66-106.56 μg·mL^-1(r=0.999 2), 7.67-122.72 μg·mL^-1(r=0.999 4), 4.88-78.08 μg·mL^-1 (r=0.999 1), 2.37-37.92 μg·mL^-1(r=0.999 1), 6.50-103.92 μg·mL^-1(r=0.999 2), 8.85-141.60 μg·mL^-1(r=0.999 4), 0.88-14.08 μg·mL^-1 (r=0.999 7), 0.74-11.92 μg·mL^-1(r=0.999 3), respectively. The recoveries ranged from 99.42%-103.10% and RSDs were all < 2.0%. The samples could be clearly classified by using CA and PCA, and the results of classification were consistent. CONCLUSION The established multi-component method is fast, accurate and reproducible. It can be used for the quality control of Xiongju Shangqing pills.