Effect of Angelicae Sinensis Radix and Paeoniae Radix Rubra in Different Compatibility on Noxious Heat with Blood Stasis Syndrome Rat Models

OBJECTIVE To study the effect of different compatibility of Angelicae Sinensis Radix and Paeoniae Radix Rubra on hemorheology and their serum TNF-α and IL-6 in noxious heat with blood stasis syndrome rat models. METHODS One hundred forty SPF SD male rats were randomly divided into 14 groups:control group, model group, high, middle and low dose of Angelicae Sinensis Radix groups, Paeoniae Radix Rubra groups, mixed-decoction of Angelicae Sinensis Radix and Paeoniae Radix Rubra groups and merging single-decoction of Angelicae Sinensis Radix and Paeoniae Radix Rubra groups. Each treatment group was administered with respective drugs by gavage for 14 consecutive days. After the first 12 days, except the control group, rats in other groups were induced by lipopolysaccharide(LPS) in combination with carrageenan(Ca), and the hemorheologyincluding whole blood viscosity, plasma viscosity, hematocrit(Hct), erythrocyte deformation index(EDI) and erythrocyte aggregation index(EAI), prothrombin time(PT), activated partial thromboplastin time(APTT) and inflammatory factors(IL-6, TNF-α) were determined. RESULTS Compared with the model group, the high and middle dose of mixed-decoction of Angelica Sinensis Radix and Paeoniae Radix Rubra could significantly decrease the whole blood viscosity under the different shear rates, plasma viscosity, EAI, Hct and EDI(P<0.05 or P<0.01), increase the PT and APTT(P<0.01), and decrease the serum level of IL-6 and TNF-α(P<0.01). CONCLUSION The mixed-decoction of Paeoniae Radix Rubra and Angelica Sinensis Radix can significantly improve the abnormality change of hemorheology and coagulation function and reduce the abnormally rise of the serum level of inflammatory factors in noxious heat with blood stasis syndrome model rats. And the therapeutic effect of the mixed-decoction of Paeoniae Radix Rubra and Angelica Sinensis Radix is superior to Paeoniae Radix Rubra or Angelica Sinensis Radix used alone.