Simultaneous Determination of Four Constituents in Feining Preparation by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and hyperin in Feining granules, Feining capsules and Feining tablets. METHODS Preparation was performed on a 30℃ Waters XBridge^TM C₁₈ column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.4% phosphoric acid flowing at 1.0 mL·min^-1 in a gradient elution manner, and the detection wavelengths were set at 327, 353 nm. RESULTS Four constituents showed good linear relationships with their own ranges(r>0.999 0), while the average recoveries were 96.1%-104.7% with the RSDs of 0.4%-1.6%. CONCLUSION This accurate, reliable and reproducible method can be used for the quality control of Feining preparation.