Effect of Pseudomonas Aeruginosa Mannose-sensitive Hemagglutinin on Proliferation of Gastric Cancer NCI-N87 Cells and Its Mechanism

OBJECTIVE To investigate the effect of Pseudomonas aeruginosa mannose-sensitive hemagglutinin (PA-MSHA) on the proliferation of gastric cancer NCI-N87 cells and its mechanism. METHODS MTT assay was used to detect the effect of PA-MSHA on the proliferation of gastric cancer cells NCI-N87. The apoptosis rate was tested by flow cytometry. The ability of cell migration and invasion was detected by wound healing assay and invasion assay. Western Blot was carried out to examine the protein expression of PTEN and p-AKT. MTT assay was used to further examine the effect of PA-MSHA on cell proliferation after PTEN siRNA transfection. RESULTS PA-MSHA inhibited the proliferation of gastric cancer cells NCI-N87 in a dose- and time-dependent manner. Compared with control group, after treatment with PA-MSHA, the apoptosis rate of NCI-N87 cells was significantly increased(P<0.05), and the migration and invasion ability of NCI-N87 cells was significantly decreased(P<0.05). Compared with control group, PA-MSHA significantly increased the expression of PTEN protein and decreased the level of p-AKT protein(P<0.05). Knock down of PTEN by siRNA transfection weakened the ability of PA-MSHA on inhibiting the proliferation of NCI-N87 cells compared with control group(P<0.05). CONCLUSION PA-MSHA can inhibit proliferation, promote apoptosis and inhibit invasion and metastasis of gastric cancer cell line NCI-N87, and its mechanism may be related to PTEN/AKT signaling pathway.