Study on the Anti-influenza Virus of Extracts from Fagopyrum Dibotrys (D. Don) Hara in Vitro

OBJECTIVE To explore the anti-influenza virus of extracts from Fagopyrum dibotrys in vitro. METHODS Hemagglutination titer test was used to investigate the inhibitory effect of extracts from Fagopyrum dibotrys on the proliferation of influenza virus in chicken embryo. The cytoactive was tested by MTT assay and investigated the effect of extracts from Fagopyrum dibotrys on the activity of infected cells by influenza virus. RESULTS Compared with the virus control group, the high dose group(10 mg·mL^-1) and the middle dose group(5 mg·mL^-1) of extracts from Fagopyrum dibotrys significantly decreased the hemagglutination titer of chicken embryo allantoic fluid(P<0.01), whlie the low dose group(2.5 mg·mL^-1) was no significant difference. In the concentration range of 0.63-2.50 mg·mL^-1, the cytoactive of the groups of extracts from Fagopyrum dibotrys were significantly higher than those in the virus control group(P<0.01), and the inhibition rate increased in a dose-dependent. The inhibition rate of maximum non-toxic concentration of extracts from Fagopyrum dibotrys(2.5 mg·mL^-1) was 66.12%. CONCLUSION The extracts from Fagopyrum dibotrys has anti-influenza effect in vitro.