Determination of Patulin in Health Products by HPLC-MS/MS with Isotope Dilution and Dispersive Solid Phase Extraction

OBJECTIVE To develop an HPLC-MS/MS with isotope dilution and dispersive solid phase extraction method to determine patulin in health products. METHODS Enzymolysis samples were extracted with acetonitrile, and then purified by a mixture of anhydrous sodium sulfate, C₁₈-N and NH₂-PSA. The analysis was performed by a HPLC-MS/MS system with Waters Atlantis-T3(3.0 mm×50 mm, 5 µm) column. The mobile phase consisted of acetonitrile and water(20:80), and multiple reaction monitoring(MRM) mode with negative electrospray ionization was used. The effect of the dosages of anhydrous magnesium sulfate, C₁₈-N and NH₂-PSA in cleaning-up agent was studied. The main influence factors and analytical conditions were modified. RESULTS The linear calibration curve was obtained with r² ≥ 0.998 5. The recoveries were ranged from 85.7% to 93.4%. RSD(n=6) ranged from 3.2% to 7.0%. The LOQ was 5.0 µg·kg^-1. CONCLUSION It is suitable for the determination of patulin in health products.