Study on Purification Process of Total Flavonoids from Caragana Altaica by the Box-Behnken Design

OBJECTIVE To study the process condition of purification the total flavonoids from Caragana altaica by macroporous adsorption resin. METHODS The Caragana altaica which collected from Shirengou in Urumqi were used as raw materials to obtain the crude flavonoids by ultrasonic extraction. The adsorption and desorption effects of seven macroporous resin on the total flavonoids in Caragana altaica were compared and optimized by Box-Behnken design. The flavonoid purification process, based on the adsorption rate and desorption rate of the resin, investigated the effects of factors such as the concentration of injection solution, injection volume, injection flow rate, the eluent, elution volume and the elution flow rate on the purification effect. RESULTS The AB-8 macroporous adsorption resin was selected as the optimum resin, and the optimal adsorption conditions and desorption conditions were obtained. The adsorption conditions were as followings:the injection concentration was 1.14 mg·mL^-1, and the injection volume was 2.8 BV. The sample flow rate was 2.5 mL·min^-1; desorption conditions:the eluent concentration was 70%, the elution volume was 2.0 BV, and the elution flow rate was 3.0 mL·min^-1. The content under this condition increased from 1.72% to 22.95%, and the purity increased by 13.3 times. CONCLUSION The purification process is simple and feasible, and provides experimental basis for the further study of total flavonoids in Caragana altaica.