Simultaneous Determination of Six Kinds of Components in Yixintong Capsules by HPLC-DAD

Objective To establish an HPLC-DAD method for determination of six bioactive components in Yixintong capsules, including puerarin, gentiopicroside, ferulic acid, acteoside, salvianolic acid B and paeonol. Methods A stable HPLC method was established and the chromatography was accomplished on a XSELECT^TM HSS C₁₈ column, the mobile phase consisted of methanol(A)-0.2% phosphoric acid(B) in a gradient mode. The flow rate was 1.0 mL·min^-1 and the column temperature was set at 30℃. The detection wavelength was set at 220 nm for paeoniflorin, acteoside and salvianolic acid B, 250 nm for puerarin, 275 nm for gentiopicroside and paeonol, respectively. Results The linear ranges of puerarin, gentiopicroside, paeoniflorin, acteoside, salvianolic acid B and paeonol were 7.56-113.4 μg·mL^-1(r=0.999 8), 4.36-65.4 μg·mL^-1(r=0.999 7), 1.18-17.7 μg·mL^-1(r=0.999 3), 1.90-28.5 μg·mL^-1(r=0.999 4), 4.48-67.2 μg·mL^-1(r=0.999 3) and 11.7-175.5 μg·mL^-1(r=0.999 7), respectively. The average recoveries were 98.7%, 97.8%, 97.6%, 98.6%, 99.0% and 98.7%, RSD were 1.0%, 1.3%, 1.4%, 1.1%, 1.2% and 1.3%. Conclusion The method is specificity, accurate and reproducible, which can be used for the quality control of Yixintong capsules.