Comparative Study on Toxicity of Aconiti Kusnezoffii Radix Cocta Formula Granule and Decoctum on H9c2 Myocardial Cell

OBJECTIVE To compare the toxicity of Aconiti Kusnezoffii Radix Cocta formula granule and decoctum on H9c2 myocardial cells under the same dosage. METHODS Different concentrations of Aconiti Kusnezoffii Radix Cocta formula granule and decoctum were applied to H9c2 myocardial cells, MTT assay was used to detect cell activity; Hoechst 33258 staining was used to detect cell nucleus morphology and semi quantitative analyze its fluorescence intensity. The release rate of lactate dehydrogenase(LDH) was analyzed by using LDH kits. RESULTS The activity of H9c2 myocardial cells decreased under the action of Aconiti Kusnezoffii Radix Cocta decoctum, the release rate of LDH increased and appeared to be concentration dependent, there was a significant difference in high concentration group with formula granule group. The cell nucleus were shrunken and fragmented under Hoechst 33258 staining, fluorescence intensity significant increased in decoctum group compared with the formula granule group under the same crude drug concentration(P<0.05). CONCLUSION The toxicity of Aconiti Kusnezoffii Radix Cocta formula granule to H9c2 myocardial cells is significantly less than its decoctum under the same crude drug concentrations.