Determination of Gypenoside XLVI and Ginsenoside Rb₃ in Gynostemma pentaphyllum by HPLC

OBJECTIVE To establish a reversed phase HPLC for simultaneous determination of gypenoside XLVI and ginsenoside Rb₃ in Gynostemma pentaphyllum. METHODS The separation of gypenoside XLVI and ginsenoside Rb₃ were performed on a Ultimate XB-C₁₈ (250 mm×4.6 mm, 5 μm) with methanol(A)-water(B) as mobile phase in a gradient elution program at a flow rate of 1 mL·min^-1. The column temperature was maintained at 30℃. The detection wavelength was 203 nm. RESULTS Gypenoside XLVI and ginsenoside Rb₃ showed a good linearity range in 3.30-39.62 μg·mL^-1 (r²=0.999 7) and 3.27-39.26 μg·mL^-1 (r²=0.999 7), respectively. The average recoveries were 113% and 102%, RSD were 1.84% and 2.82%, respectively. CONCLUSION This method is accurate with high simplify, stability, and reliability, and can be used for quality research of Gynostemma pentaphyllum.