Antitumor Effect and Synergistic Effect with Cisplatin of Zedoray Turmeric Oil on Nude Mice Bearing Ovarian Cancer

OBJECTIVE To investigate antitumor effect of zedoray turmeric oil(ZTO) on nude mice bearing ovarian cancer and explore its synergistic effect with cisplatin. METHODS After the establishment of HO-8910PM tumor-bearing mice model, the mice were randomly divided into four groups:model control group(normal saline); ZTO group (300 mg·kg^-1); cisplatin group (2 mg·kg^-1); combination group(300 mg·kg^-1 ZTO+2 mg·kg^-1 cisplatin). The nude mice were sacrificed after three weeks of treatment, tumor volume in each group were detected and tumor inhibition rate were calculated. The expression of NM23, TNF-α, VEGF, NF-KBP65, PCNA in tumor tissues were determined by immunohistochemical. ELISA analysis was performed to evaluate the serum level of IL-2 and IFN-γon in nude mice. RESULTS Compared with model group, the tumor volume were significantly decreased in ZTO, cisplatin and combination group(P<0.05 or P<0.01). And the tumor inhibition rates were 20.55%, 52.52%, 55.06%, respectively. Immunohistochemical results showed that the expression of NM23, TNF-α, VEGF, NF-KBP65, PCNA could be significantly decreased with the administration of ZTO, cisplatin, ZTO and cisplatin combined (P<0.05 or P<0.01), and the tendency from low to high were ZTO, cisplatin, ZTO and cisplatin combined in turn. The levels of IL-2 and IFN-γ were also significantly increased with treatment of cisplatin as well as ZTO and cisplatin combined (P<0.01), and the tendency from low to high was the same to above. CONCLUSION ZTO has the anti-tumor effect on ovarian cancer, the underlying mechanism maybe related with regulation of the expression of tumor growth cytokine NM23, TNF-α, VEGF, NF-KBP65, PCNA and immune-related factors IL-2, IFN-γ. And ZTO combined with cisplatin have potential synergistic attenuated the antitumor effect in nude mice of ovarian cancer.