Simultaneous Determination of Verbascoside, Paeoniflorin and Baicalin in Kuntai Capsules by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of verbascoside, paeoniflorin and baicalin in Kuntai capsules, provide reference for the improvement of the existing quality standards. METHODS Using Phenomsil C₁₈(4.6 mm×250 mm, 5 μm) chromatography column; mobile phase was acetonitrile(A)-0.1% acetic(B) with gradient elution; flow rate was 1.0 mL·min^-1; column temperature was 35℃; the detection wavelength of verbascoside, paeoniflorin and baicalin was 334, 230, 280 nm, respectively. RESULTS Verbascoside had good linear relationship in 3.27-32.68 μg·mL^-1, the average recovery was 98.4%, RSD was 0.68%(n=6). Paeoniflorin had good linear relationship in 17.65-176.51 μg·mL^-1, the average recovery was 99.2%, RSD was 1.08%(n=6). Baicalin had good linear relationship in 48.78-487.77 μg·mL^-1, the average recovery was 97.7%, RSD was 0.87%(n=6). CONCLUSION The method is simple, shortcut, accurate, repeatable and can be used for the quality control of Kuntai capsules.