Development of Chlorpyrifos Test Strip Base on Bifunctional Fluorescent Nanoparticles Immune Chromatography Technology

OBJECTIVE To make chlorpyrifos test strip for on-site rapid screening and identification of chlorpyrifos in traditional Chinese medicine. METHODS CdTe@SiO2 fluorescent nanoparticle conjugated covalently with monoclonal antibody against chlorpyrifos was employed in the development of lateral flow strip assay by using the immunofluorescence technique. RESULTS This rapid test strip had a detection limit as low as 0.25 ng·kg^-1. It was easy of use and only takes 15-20 min to finish the test. CONCLUSION It is very simple in operation, high in sensitivity and specificity, and can be widely used in Chinese medicine screening and detection of chlorpyrifos residue.