Simultaneous Determination of Three Components in Qinggan Chuanxinlian Tablet by HPLC-DAD

OBJECTIVE To establish an HPLC method for simultaneous determination of andrographolide, dehydroandrographolide and isorhapontigenin in Qinggan Chuanxinlian tablet, and to provide the evidence for its quality control. METHODS The HPLC-DAD analysis was carried out by Agilent Extend C₁₈ column (4.6 mm×250 mm, 5 μm) with acetonitrile(A)-water solution(B) as the mobile phase for gradient elution. The detection wavelength was 225 nm for andrographolide, 254 nm for dehydroandrographolide, and 323 nm for isorhapontigenin, respectively. In addition, the column temperature was 35, and the flow rate was 0.8℃ mL·min^-1. RESULTS The linear range was 8.395-335.790 μg·mL^-1 for andrographolide(r=0.999 9), 5.948-237.924 μg·mL^-1 for dehydroandrographolide(r=0.999 9) and 0.854-25.607 μg·mL^-1 for isorhapontigenin(r=0.999 9); the average recoveries were 97.2%, 102.1% and 98.2%, and RSD were 1.7%, 3.0%, 1.0%, respectively. The contents of three components in 14 batches from 2 manufactories were 0.80-2.43, 0.59-2.71, and 0.045-0.275 mg per tablet. CONCLUSION This method is accurate and reliable, and it is suitable for the quality control of Qinggan Chuanxinlian tablet.