Effect of Qianggu Yin Combined with Probiotics on Osteoporotic Fracture Healing in Rats

OBJECTIVE To study the effect of Qianggu Yin combined with probiotics on osteoporotic fracture healing in rats. METHODS Fifty female Sprague-Dawley rats were randomly divided into 5 groups:fracture control group, osteoporotic fracture model group, Qianggu Yin group, probiotics group and Qianggu Yin combined with probiotics group, 10 rats in each group. Osteoporotic fracture model group and all treatment groups of osteoporotic fracture were bilaterally ovariectomized osteoporosis model preparation. After 6 weeks, each group was prepared right middle femoral fracture model. Rats in the treatment group of osteoporotic fracture were given 102 g·kg^-1 Qianggu Yin or 12.5 g·kg^-1 probiotics with intragastric administration, while rats in the fracture group and osteoporotic fracture group were given equal volume of normal saline 1 time a day for 6 weeks with intragastric administration. The bone mineral density was measured by bone mineral density scanner, and Micro-CT was used to scan femur related parameters in rats. The bone alkaline phosphatase(BALP), the osteocalcin (OC), type I procollagen carboxy-terminal propeptide(CTX-I) contents were measured by ELISA kit, calcium(Ca) concentration was measured by biochemical analyzer. HE staining was used to observe the pathological changes of periosteum in fractures. RESULTS Compared with the osteoporotic fracture group, Qianggu Yin or probiotic group could promote fracture healing(increased trabecular number and bone density), increase bone formation parameters(BALP and OC) and reduce absorption parameters(CTX-I). Qianggu Yin combined with probiotic group expressed more obvious effect of treatment. CONCLUSION Both Qianggu Yin and probiotic showed the therapeutic effect on osteoporotic fractures, and the combination of drugs has a better effect.