Rapid Determination of Luteolosided and Verbascoside in Callicarpa Nudiflora Granule Based on Near-infrared Spectroscopy

OBJECTIVE To establish a rapid detection method to detect the content of luteolosided and verbascoside in Callicarpa Nudiflora granule by near infrared spectrum. METHODS Determined the content of luteolosided and verbascoside in Callicarpa Nudiflora granule by HPLC. And used near infrared spectroscopy combining with partial least squares(PLS) to set up quantitative calibration model of luteolosided and verbascoside. RESULTS The correlation coefficients(r) of the quantitative correction model of luteolosided and verbascoside were 0.987 9 and 0.956 3 respectively. The values of root mean square error of cross validation(RMSECV) were 0.004 6 and 0.063 7. The correlation coefficient(r) of the verification set were 0.905 8 and 0.964 8 respectively. The mean square error(RMSEP) were 0.020 0 and 0.079 8. CONCLUSION Based on near infrared spectroscopy, the study establish a rapid quantitative model of luteolosided and verbascoside and its performance is good, and the prediction results of the model are good. This study provides a scientific and reasonable method for the content determination and quality control of Chinese medicine granules.