Protective Effect of Novel Gastrodin Derivatives on Alzheimer's Disease Model Mice

OBJECTIVE To investigate the protective effect of ferulic acid(FA), gastrodin(Gas) and gastrodin derivative(Gas-D) in an Alzheimer's disease mouse model induced by bilateral intracerebroventricular injection of amyloid-β oligomers(AβOs). METHODS C57BL/6 mice were randomly divided into 5 groups:sham group, model group, FA group, Gas group and Gas-D group. After the bilateral lateral ventricle was injected with AβOs to establish AD model mice, continuous intragastric administration for 14 d. Y maze test and step down test were used to evaluate the memory ability. The expressions of astrocytes and microglial cells were determined by immunofluorescence. The mRNA levels of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), superoxide dismutase 2(SOD2) and catalase(CAT) in brain of mice were measured by qRT-PCR. RESULTS Compared with model group, FA, Gas and Gas-D groups could significantly improve the ability of memory of mice, reduce the inflammation and oxidative damage of brain tissues. Furthermore, compared with FA and Gas group, Gas-D group also had significant differences. CONCLUSION Compared with FA and Gas, Gas-D is more effective to alleviate the memory loss, inflammation and oxidative damage induced by AβOs. Thus, it provides a clinic application potential of Gas-D as a therapy of AD.