Role of GM-CSF and Mobilized Bone Marrow-derived Cells in Wound Healing

OBJECTIVE To investigate the role of GM-CSF and its mobilized bone marrow-derived cells in promoting wound healing. METHODS The myelosuppressive mice model induced by chemotherapy and radiotherapy was established. The wounds were formed on the back and subcutaneously injected with 50, 17 μg·kg^-1 GM-CSF to measure the degree of wound healing. MTT was used to observe the proliferation of PIECs. The cultured human umbilical vein endothelial cells and mouse arteries were cultured with mateigel, and 100 ng·mL^-1 GM-CSF was administered to observe the formation of microtubules. RESULTS In the mouse wound healing model, wound repair of GM-CSF (50 μg·kg^-1) was more obvious than GM-CSF (17 μg·kg^-1). A low dose of GM-CSF(17 μg·kg^-1) promoted wound healing in myelosuppressed mice. GM-CSF at 0.01-781.25 ng·mL^-1 significantly promoted the proliferation of PIECs. GM-CSF 100 ng·mL^-1 significantly promoted the formation of microtubules around human umbilical vein endothelial cells and arterial rings. CONCLUSION GM-CSF promotes wound healing by mobilizing bone marrow-derived cells and acting on endothelial cells.