Isolation and Identification of Chemical Constituents from Twigs and Leaves of Evodia lepta (Spreng.) Merr

OBJECTIVE To study the chemical constituents from the twigs and leaves of Evodia lepta (Spreng.) Merr.. In order to lay a substantial foundation for the further research. METHODS The ethyl acetate fraction of anhydrous ethanol extract from Evodia lepta (Spreng.) Merr. was separated and purified by silica gel column chromatography. The structures of the compounds were identified by nuclear magnetic resonance (NMR) spectrometry. RESULTS Six compounds were isolated as isoevodionol (compound 1), isoevodionol methyl ether(compound 2), 3,5-dihydroxy-4-ethoxy-6-acetyl-7-methoxy-2,2-dimethylchroman (compound 3),(cis)-3,4,5-trihydroxy-6-acetyl-7-methoxy-2,2-dimethylchroman(compound 4), (trans)-3,4-dihydroxy-5-methoxy-6-acetyl-7-methoxy-2,2-dimethylchroman (compound 5), butylparaben (compound 6). CONCLUSION The compounds(compound 1-6) are isolated from the twigs and leaves of Evodia lepta (Spreng.) Merr. for the first time.