Separation of Nefopam Hydrochloride with a Bonded Cellulose Chiral Stationary Phase

OBJECTIVE To develop an HPLC method with a bonded cellulose chiral stationary phase for the chiral separating enantiomers of nefopam hydrochloride. METHODS Nefopam hydrochloride was separated by a Chiralpak IC (250 mm×4.6 mm, 5 μm) at 25℃ and detected at 267 nm, the mobile phase was n-hexane-isopropanol(90:10, 0.1% DEA) at a flow rate of 0.8 mL·min^-1. RESULTS Nefopam hydrochloride enantiomers were separated on a cellulose bonded chiral stationary phase and the resolution was 6.53. CONCLUSION The method can be used for quickly separating the enantiomers of nefopam hydrochloride.