Identification of Various Chemical Constituents in Dandeng Tongnao Capsule by UHPLC-Q-Orbitrap HRMS

OBJECTIVE To identify the chemical constituents in Dandeng Tongnao capsule by ultra high performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry(UHPLC-Q-Orbitrap HRMS). METHODS The ACQUITY UPLC^® BEH C₁₈ chromatographic column(2.1 mm×50 mm, 1.7 μm) was used for UPLC, and the acetonitrile-0.1% formic acid water was used as mobile phase to separate various chemical constituents by gradient elution separation. Chromatographic effluent was detected by UHPLC-Q-Orbitrap HRMS full scan in positive and negative ion mode. According to precise molecular weight of compounds and multistage fragment ion information detected by UHPLC-Q-Orbitrap HRMS, reference and database information, it could be analyzed accurate characterization of the compound compared with relative retention time and mass spectrometry data of control sample. RESULTS Fifty-nine chemical constituents which mainly including flavonoids, quinones, and organic acids were identified and classified. CONCLUSION A variety of chemical constituents can be accurately identified by UHPLC-Q-Orbitrap HRMS. The results can be consulted by the further study of the pharmacodynamic basis and quality standard of Dandeng Tongnao capsule.