Determination of 16 Anti-allergic Drugs in Chinese Traditional Patent Medicine and Health Food by UPLC

OBJECTIVE To develop a UPLC method to determine 16 anti-allergic drugs illegally added in chinese traditional patent medicine and health food. METHODS The separation was performed on a Waters BEH C₁₈ column (100 mm×2.1 mm, 1.7 μm) with the mobile phase consisted of methanol-20 mmol·L^-1 sodium dihydrogen phosphate solution (adjusted to pH 3.0 with phosphoric acid) in a gradient elution mode, the detector wavelength was 220 nm. RESULTS The results of 16 anti-allergic drugs showed good linearity in the range of 2.5-40 μg·mL^-1(r²>0.999). The average recoveries were from 90.77% to 113.35%, with the RSD from 0.06% to 1.21%. The limits of quantifications were among 0.18-3.92 μg·mL^-1, and the limits of detections were among 0.09-0.78 μg·mL^-1. CONCLUSION This method is simple and sensitive, and it can effectively judge whether the chinese patent medicines and health products are illegally mixed with these 16 anti-allergic drugs.