Study on the Antitoxic Effect of Self-microemulsifying Drug Delivery System on Triptolide

OBJECTIVE To investigate the hepatotoxicity and renal toxicity of self-microemulsifying drug delivery system containing triptolide(TRL-SMEDDS) in mice.METHODS MGC80-3 human gastric tumor model in nude mice was constructed, and the mice were divided into model control group, triptolide(TRL) crude drug group and TRL-SMEDDS group. The mice were fed with saline, free TRL and TRL-SMEDDS, respectively, for once/2 days in 23 d. After 24 h of the last treatment, the serum of nude mice in each group were collected, and the level of ALT, AST, Urea, Scr, STP, ALB, GLOB and UA in serum were determined. The animals were weighed and the liver and kidney were also collected. The appearance and morphology of the liver and kidney were observed by the biological microscope after pathological section staining, and the liver and kidney index were calculated. RESULTS Compared with free TRL crude drug group, the level of AST and ALT in TRL-SMEDDS group was significantly declined(P<0.01), STP level was increased significantly(P<0.05), and A/G was also significantly rose (P<0.01). Scr level showed slightly decrease but had no statistical significance. Urea and UA level were decreased significantly(P<0.05 and <0.01, respectively). In TRL-SMEDDS group, liver cells were slightly swollen and a few inflammatory cells were found in liver tissue. In TRL crude drug group, some steatosis and fragmentation necrosis were showed in liver tissue. The hepatocyte nucleus obviously enlarged, and a large number of fibrous tissue was found. There were no obvious abnormal pathological features in renal tissue of both groups. CONCLUSION The TRL-SMEDDS presents a significantly decrease of hepatotoxicity and renal toxicity compared with free TRL.