Study on the Effect of bFGF Combined with Antrodia Camphorate Polysaccharide on the Repair of Neural Function After Mechanical Spinal Cord Injury

OBJECTIVE To study the effect of bFGF combined with Antrodia camphorate polysaccharide on the repair of neural function after mechanical spinal cord injury. METHODS The mechanical spinal cord injury rats model was established by modified Allens method. The rats were divided into control group, model group, bFGF group, polysaccharide group and bFGF+polysaccharide group. The drug intervention time was 30 days. The Basso-Beattie-Bresnahan (BBB) score, somatosensory evoked potential (SEP) test and motor evoked potential (MEP) experiment were performed on 1, 7, 14, 21, 30 d after drug intervention. After 30 d, the rats were executed for HE staining and Nissl staining with spinal cord tissue. The expression of TNF-α, IL-1β, CXCL-10, GM-CSF and IL-10 in the tissue were detected by Elisa. RESULTS BFGF and polysaccharides alone were superior to model group in BBB score and SEP and MEP test results(P<0.05), the combined group (bFGF+polysaccharide) in BBB score and SEP and MEP test were better than those of the single medication group(P<0.05). The expression of inflammatory factors TNF-α, IL-1β, and chemokine CXCL-10 in spinal cord tissue of bFGF group, polysaccharide group and bFGF+polysaccharide group were significantly lower than those of model group(P<0.05). The expression of GM-CSF and IL-10 was higher than that of model group(P<0.05). The bFGF+polysaccharide group was significantly better than the bFGF group and the polysaccharide group(P<0.05). CONCLUSION bFGF combined with Antrodia camphorate polysaccharide can improve the neural function in mechanical spinal cord injury, the mechanism may be related to the expression of anti-inflammatory factors and the survival of nerve cells.