Study on HPLC Characteristic Spectrum of Jiangzhi Qingshen Tea and Determination of Four Active Components

OBJECTIVE To establish an HPLC characteristic spectrum of Jiangzhi Qingshen tea and simultaneous determination of four active components. METHODS With chlorogenic acid, rutin, isoquercitrin and kaempferol-3-O -rutinoside as reference substance, the HPLC analysis was carried on an Agilent Zorbax SB-C₁₈ column (4.6 mm×250 mm, 5 μm) with the column temperature of 40℃; the mobile phase was acetonitrile-0.2% phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL·min^-1; the detection wavelength was 360 nm. The similarity evaluation system for chromatographic fingerprint of TCM was used to calculate the similar degree. RESULTS The linear ranges of chlorogenic acid, rutin, isoquercitrin, and kaempferol-3-O-rutinoside were 0.072 6-0.726, 0.076 3-0.763, 0.071-0.71, 0.113 1-1.131 μg, respectively (r ≥ 0.999 6), and the average recoveries of three components were 96.15%, 95.62%, 95.28%, 95.76%, respectively. CONCLUSION With a good correlation, the characteristic spectrum can be combined with the assay of 4 components to control the quality of Jiangzhi Qingshen tea comprehensively.