Anti-inflammatory Activity of Swertiamarin and Its Effect on the Expression of NF-κB Pathway-related Factors p65 and IKK-α

OBJECTIVE To observe the anti-inflammatory activity of Swertiamarin and its regulation effect on the nuclear transcription factor-κB(NF-κB) pathway. METHODS Lipopolysaccharid(LPS) induced murine macrophage raw 264.7 cell was used to establish an inflammatory model in vitro. CCK-8 method was used to observe the effect of Swertiamarin on cell activity. ELISA was used to detect its effect on the expression of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6). RT-PCR was used to detect its effect on mRNA expression of NF-κB pathway related factors p65 and Ikk-α. RESULTS The 100 μg·mL^-1 Swertiamarin could decrease the cell survival rate, and 3.125-50 μg·mL^-1 had no effect on the cell survival rate. The 25-50 μg·mL^-1 Swertiamarin could reduce the release of TNF-α and IL-6. It was found that 12.5-50 μg·mL^-1 Swertiamarin could inhibit the mRNA expression of p65, and the mRNA expression of Ikk-α could be inhibited by 50 μg·mL^-1 Swertiamarin. CONCLUSION Swertiamarin has inhibitory effect on TNF-α and IL-6 production in LPS-induced inflammatory model, and its mechanism may be related to the inhibition of the expression of p65 and IKK-α, which are related factors of NF-κB pathway.