Simultaneous Determination of Five Active Components in Xiaoyanlidan Tablets by HPLC

OBJECTIVE To establish a method for simultaneous of chlorogenic acid, rosmarinic acid, andrographolide, apigenin and dehyandrographolide in Xiaoyanlidan tablets by HPLC. METHODS The HPLC separation was achieved on a phenomonex^®-C₁₈ (4.6 mm×250 mm, 5 μm) column. The mobile phase consisted of acetonitrile (A) and 0.4%phosphoric acid (B) solution with gradient elution. The flow rate was 1.0 mL·min^-1. The column temperature was 30℃. The detection wavelength was set at 330 nm(chlorogenic acid, rosmarinic acid and apigenin) and 254 nm (andrographolide, dehyandrographolide). RESULTS The linear range of chlorogenic acid, rosmarinic acid, andrographolide, apigenin and dehyandrographolide were 3.042-121.7 μg·mL^-1, 2.558-102.3 μg·mL^-1, 14.11-564.4 μg·mL^-1, 2.835-113.4 μg·mL^-1, 23.86-954.3 μg·mL^-1 respectively (r ≥ 0.999 6); the average recoveries were 97.90%-101.75% and the corresponding RSD was 0.89%-1.66% respectively. The precision, repeatability and stability were good. CONCLUSION The established method is accurate, reliable and repeatable, which can provide a more comprehensive basis for quality control of Xiaoyanlidan tablets.