UPLC-ELSD Fingerprints of Fritillariae Pallidiflorae Bulbus of Wild Growing and Cultivated Species from Different Habitats

OBJECTIVE To establish a UPLC-ELSD fingerprint method for quality control of Fritillariae Pallidiflorae Bulbus. METHODS Waters Acquity UPLC^TM BEH C₁₈ chromatographic column (100 mm×2.1 mm, 1.7 μm) was adopted for gradient elute with the mobile phase consisting of acetonitrile-0.02% triethylamine-water. The flow rate was 0.25 mL·min^-1; the temperature of column was set at 25℃, sample temperature was room temperature and the Drift tube temperature was 40℃, spray parameter was 40%, the gain value was 500, the gas pressure was 30 psi. Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2012 A) published by the State Pharmacopeia Committee of China was employed for the representative standard fingerprint, and the similarity of each chromatogram of Fritillariae Pallidiflorae Bulbus of wild and cultivated species from different habitats was also calculated by the two methods. Hierarchical cluster analysis was applied to investigating the test data. RESULTS The chromatographic fingerprint showed 16 characteristic peaks, which was established from 29 species of Fritillariae Pallidiflorae Bulbus and was generated as the representative standard fingerprint. The similarity of the chromatographic fingerprints from the 29 samples was ≥ 0.801. Twenty-nine samples were classified into 2-3 groups. And the contents of sipeimine-3β-D-glucoside and sipeimine were determined from 29 wild and cultivated species of Fritillariae Pallidiflorae Bulbus from different habitats. CONCLUSION The method of UPLC-ELSD fingerprint is time-saving and can be used for the comprehensive quality evaluation of Fritillariae Pallidiflorae Bulbus.