Study on the Plasma Protein Combine Rate of the Active Compounds of Aconiti Lateralis Radix Praeparata by HPCE

OBJECTIVE To establish a method for evaluating the plasma protein binding rate of alkaloid in Aconiti Lateralis Radix Praeparata. METHODS The chromatography condition such as sample injection condition and the ingredient of electrophoretic buffer solution were evaluated. And the plasma protein binding rate of benzoylhypaconine, benzoylaconine and benzoylmesaconine were analyzed. RESULTS The chromatography condition was as followes:voltage injection, electrophoretic buffer solution100 mmol·L^-1 borate saline buffer (pH=9.0):menthol=7:3, separation voltage was 15 kV, the detection wavelength was 230 nm. Within the level of 1, 2, 5 μg·mL^-1, the plasma protein binding rates of benzoylhypaconine were (43.81±3.2)%, (42.51±3.6)%, (42.09±2.3)%; the plasma protein binding rates of benzoylaconine were (39.59±4.0)%, (34.58±3.7)%, (37.02±3.0)%; and the plasma protein binding rates of benzoylmesaconine were (48.23±4.6)%, (33.26±3.9)%, (32.60±3.8)%. CONCLUSION Ultrafiltration combined with HPCE technology can be used as a new method for the analysis of plasma protein binding rate.