Study on the Antifatigue Effect of Calf Spleen Extractive Injection on Mice

OBJECTIVE To evaluate the antifatigue effect of Calf Spleen Extractive injection (CSEI) on mice and to explore its preliminary mechanism. METHODS Exercise tolerance test, fatigue and oxidation related biochemical indices and plasma hormone level were detected with Thymosinα1 (Tα1) served as control drug. RESULTS After 21 d of intraperitoneal administration, CSEI improved the running and loading swimming time of mice significantly. CSEI could reduced the LD (in plasma, liver and muscle) and LDH (in liver and muscle) levels simultaneously, and increased the liver glycogen and muscle glycogen. Meanwhile, CSEI improved the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) levels in plasma, liver and muscle, decreased reactive oxygen species (ROS) level both in liver and muscle, and the variation of plasma hormone testosterone (T) and cortisol was also viewed. CONCLUSION CSEI display an antifatigue effect possibly through antioxidant and hormone regulation.