Optimization and Identification of Anti-sepsis Potential Targets of Palmatine

OBJECTIVE To study the characteristics of palmatine, to optimize and identify the potential targets of palmatine against sepsis. METHODS TCMSP database were used to analysis the pharmacological parameters and molecular characteristics of palmatine; TCMSP and DRAR-CPI software were used to select the anti-sepsis potential targets of palmatine; molecular docking software and immunohistochemical method were used to identify the anti-sepsis potential target. RESULTS The oral bioavailability of palmatine was 64.60%, the blood-brain barrier permeability was 0.37, and the drug-likeness was 0.65, indicating that palmatine had good drug formation. Three potential targets were optimized by TCMSP and DRAR-CPI software. NOS3 was identified as the potential target for anti-sepsis of palmatine by molecular docking software, the immunohistochemical result showed that palmatine could significantly down-regulate the expression of NOS3 in septic rats. CONCLUSION Palmatine has good drug formation, the anti-sepsis effect of it may be through binding and activating NOS3 to protect the myocardial inhibition.