Determination of Eburicoic Acid and Total Tritepenoidic from Fomes Officinalis Ames

OBJECTIVE To determine the content of total tritepenoidic and eburicoic acid from Fomes officinalis Ames. METHODS The content of total tritepenoidic was determined by ultraviolet spectrophotometry. The content of eburicoic acid was determined by HPLC. The analysis was performed on a RP-C₁₈ column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.4% phosphoric acid (68:32) as mobile phase at a flow rate of 1.0 mL·min^-1, and temperature was 30℃ The detection of wavelength was set at 210 nm. RESULTS The contents of eburicoic acid in 3 batches of Fomes officinalis Ames were 2.6%, 2.3%, 3.1%, and the contents of total tritepenoidic were 32.1%, 27.2%, 35.9%, respectively. CONCLUSION The method is suitable for the determination of tritepenoidic components from Fomes officinalis Ames.