Simultaneous Determination of Four Constituents in Imported Panax Quinquefolium L. by UHPLC

OBJECTIVE To establish a UHPLC method for simultaneous determination of four active components (ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ and ginsenoside Rd) in imported Panax quinquefolium L.. METHODS An Agilent Zorbax SB C₁₈ column (2.1 mm×100 mm, 1.8 μm) was used, with mobile phase of acetonitrile as mobile phase A and water as mobile phase B (gradient elution) at a flow rate of 0.5 mL·min^-1, column temperature was 30℃ and detector wavelength was 203 nm. RESULTS The four components showed good separation and linearity within the tested range. The mean recoveries were in the range of 95.7%-100.3% (RSD 1.4%-1.8%). CONCLUSION It provides a rapid and reliable UHPLC method which is suitable for the quality control of Panax quinquefolium L..