Isolation and Elucidation of Casticin from Uighur Medicine Artemisia Rupestris and Its Quantitative Analysis

OBJECTIVE To study isolation and elucidation of casticin from Uighur medicine Artemisia rupestris and its quantitative analysis. METHODS Casticin was isolated and enriched by HPD-450 macroporous absorption resin with silica gel column and Sephadex LH-20 column chromatography. Its structure was elucidated by spectral analysis. HPLC was used to determine the content of casticin from Uighur medicine Artemisia rupestris. The Agilent HC-C₁₈(250 mm×4.6 mm, 5 μm) column was used with mathnol-warter (70:30) as the mobile phase at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 350 nm. The column temperature was 30℃. RESULTS the casticin content in purified product was up to 99.7%. Casticin was linear over the ranges of 9.42-75.36 μg·mL^-1(r=0.999 6), and the average recovery rate was 109.2%, RSD was 2.12%. CONCLUSION Highly purified casticin may be obtained effectivly by macroporous absorption resin method. The HPLC method for determination the content of casticin is simple, accurate and steady. It can be used for the quality control of Uighur medicine Artemisia rupestris.