Simultaneous Determination of Irisflorentin and Piperine in Danlyu Bushen Capsules by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination of irisflorentin and piperine in Danlyu Bushen capsules. METHODS The HPLC was carried on Agilent ZORBAX Eclipse Plus C₁₈(4.6 mm×250 mm, 5 μm), the mobile phase acetonitrile-0.2% phosphoric acid (41:59), the flow rate was 1.0 mL·min^-1, the column temperature was 40℃, detection wavelength was 266 nm. RESULTS Under the chromatographic conditions, irisflorentin in 0.010 30-0.309 0 μg (r=0.999 8); piperine in 0.081 73-2.451 9 mg (r=0.999 8), had good linear relationship. Irisflorenti and piperine average sample recovery rate (n=9) were 99.7% and 100.6%; RSD were 1.2%, 0.9%, respectively. In the sample, the minimum content of irisflorentin and piperine was 0.35, 6.4 mg per granule, respectively. CONCLUSION The method is simple, accurate and has a good repeatability. It can be used as the quality control method of Danlyu Bushen capsules.